Hydrodynamic characterization of a new small-scale reactor mixed by a magnetic bar
Identifieur interne : 000151 ( Main/Exploration ); précédent : 000150; suivant : 000152Hydrodynamic characterization of a new small-scale reactor mixed by a magnetic bar
Auteurs : Charline Allonneau [France] ; Eric Olmos [France] ; Stéphane Guyot [France] ; Eric Ferret [France] ; Patrick Gervais [France] ; Rémy Cachon [France]Source :
- Biochemical Engineering Journal [ 1369-703X ] ; 2015-04.
Abstract
The purpose of this study was to evaluate the mixing characteristics of a new milliliter-scale (mL-scale) reactor developed for studying enzymatic activity or physiological cell response. The mL-scale reactor was designed to enable the integration of several sensors to carry out dynamic measurements in a controlled environment. Rapid homogeneity of the entire system is essential to ensure reproducible and reliable results, consequently the reactor was stirred to optimize both mass and heat transfers. A comparative study using three different techniques was undertaken to study mixing performances in the system. Firstly, mixing time (4,) was estimated in the reactor using both experimental methods, including instrumental method and image analysis, and CFD. As hydrodynamics is not occurring in the fully-turbulent regime, turbulent numerical simulations using the SST transitional and the standard k-epsilon turbulence models were conducted and compared to laminar approach. Results showed good agreement between the two experimental methods and no significant differences were observed between the simulation methods. Moreover, according to results, a minimum agitation rate of 350 rpm seemed to be appropriated to obtain a quick homogenization in the system. Finally, the second part of the study seemed to indicate that probes had no significant impact in the studied reactor.
Url:
DOI: 10.1016/j.bej.2014.12.005
Affiliations:
- France
- Grand Est, Lorraine (région), Région Bourgogne
- Dijon, Metz, Nancy
- Université de Bourgogne, Université de Bourgogne Franche-Comté, Université de Lorraine
Links toward previous steps (curation, corpus...)
- to stream Hal, to step Corpus: 000576
- to stream Hal, to step Curation: 000576
- to stream Hal, to step Checkpoint: 000145
- to stream Main, to step Merge: 000151
- to stream Main, to step Curation: 000151
Le document en format XML
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1 esplanade Erasme - 21000 Dijon</addrLine><country key="FR"></country></address><ref type="url">http://www.umr-pam.fr</ref></desc><listRelation><relation active="#struct-300270" type="direct"></relation><relation name="UMR MA AGROSUP 2012.02.102" active="#struct-415469" type="direct"></relation></listRelation><tutelles><tutelle active="#struct-300270" type="direct"><org type="institution" xml:id="struct-300270" status="VALID"><orgName>Université de Bourgogne</orgName><orgName type="acronym">UB</orgName><desc><address><addrLine>Maison de l'université - Esplanade Érasme - BP 27877 - 21078 Dijon cedex</addrLine><country key="FR"></country></address><ref type="url">http://www.u-bourgogne.fr/</ref></desc></org></tutelle><tutelle name="UMR MA AGROSUP 2012.02.102" active="#struct-415469" type="direct"><org type="institution" xml:id="struct-415469" status="VALID"><orgName>Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement</orgName><orgName type="acronym">AgroSup Dijon</orgName><desc><address><country key="FR"></country></address><ref type="url">http://www.agrosupdijon.fr/</ref></desc></org></tutelle></tutelles></hal:affiliation><country>France</country><placeName><settlement type="city">Dijon</settlement><region type="region" nuts="2">Région Bourgogne</region></placeName><orgName type="university">Université de Bourgogne</orgName><orgName type="institution" wicri:auto="newGroup">Université de Bourgogne Franche-Comté</orgName></affiliation></author><author><name sortKey="Cachon, Remy" sort="Cachon, Remy" uniqKey="Cachon R" first="Rémy" last="Cachon">Rémy Cachon</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-189516" status="VALID"><orgName>Procédés Alimentaires et Microbiologiques</orgName><orgName type="acronym">PAM</orgName><date type="start">2012-01-01</date><desc><address><addrLine>Université de Bourgogne - 1 esplanade Erasme - 21000 Dijon</addrLine><country key="FR"></country></address><ref type="url">http://www.umr-pam.fr</ref></desc><listRelation><relation active="#struct-300270" type="direct"></relation><relation name="UMR MA AGROSUP 2012.02.102" active="#struct-415469" type="direct"></relation></listRelation><tutelles><tutelle active="#struct-300270" type="direct"><org type="institution" xml:id="struct-300270" status="VALID"><orgName>Université de Bourgogne</orgName><orgName type="acronym">UB</orgName><desc><address><addrLine>Maison de l'université - Esplanade Érasme - BP 27877 - 21078 Dijon cedex</addrLine><country key="FR"></country></address><ref type="url">http://www.u-bourgogne.fr/</ref></desc></org></tutelle><tutelle name="UMR MA AGROSUP 2012.02.102" active="#struct-415469" type="direct"><org type="institution" xml:id="struct-415469" status="VALID"><orgName>Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement</orgName><orgName type="acronym">AgroSup Dijon</orgName><desc><address><country key="FR"></country></address><ref type="url">http://www.agrosupdijon.fr/</ref></desc></org></tutelle></tutelles></hal:affiliation><country>France</country><placeName><settlement type="city">Dijon</settlement><region type="region" nuts="2">Région Bourgogne</region></placeName><orgName type="university">Université de Bourgogne</orgName><orgName type="institution" wicri:auto="newGroup">Université de Bourgogne Franche-Comté</orgName></affiliation></author></analytic><idno type="DOI">10.1016/j.bej.2014.12.005</idno><series><title level="j">Biochemical Engineering Journal</title><idno type="ISSN">1369-703X</idno><imprint><date type="datePub">2015-04</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The purpose of this study was to evaluate the mixing characteristics of a new milliliter-scale (mL-scale) reactor developed for studying enzymatic activity or physiological cell response. The mL-scale reactor was designed to enable the integration of several sensors to carry out dynamic measurements in a controlled environment. Rapid homogeneity of the entire system is essential to ensure reproducible and reliable results, consequently the reactor was stirred to optimize both mass and heat transfers. A comparative study using three different techniques was undertaken to study mixing performances in the system. Firstly, mixing time (4,) was estimated in the reactor using both experimental methods, including instrumental method and image analysis, and CFD. As hydrodynamics is not occurring in the fully-turbulent regime, turbulent numerical simulations using the SST transitional and the standard k-epsilon turbulence models were conducted and compared to laminar approach. Results showed good agreement between the two experimental methods and no significant differences were observed between the simulation methods. Moreover, according to results, a minimum agitation rate of 350 rpm seemed to be appropriated to obtain a quick homogenization in the system. Finally, the second part of the study seemed to indicate that probes had no significant impact in the studied reactor.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Grand Est</li><li>Lorraine (région)</li><li>Région Bourgogne</li></region><settlement><li>Dijon</li><li>Metz</li><li>Nancy</li></settlement><orgName><li>Université de Bourgogne</li><li>Université de Bourgogne Franche-Comté</li><li>Université de Lorraine</li></orgName></list><tree><country name="France"><region name="Région Bourgogne"><name sortKey="Allonneau, Charline" sort="Allonneau, Charline" uniqKey="Allonneau C" first="Charline" last="Allonneau">Charline Allonneau</name></region><name sortKey="Cachon, Remy" sort="Cachon, Remy" uniqKey="Cachon R" first="Rémy" last="Cachon">Rémy Cachon</name><name sortKey="Ferret, Eric" sort="Ferret, Eric" uniqKey="Ferret E" first="Eric" last="Ferret">Eric Ferret</name><name sortKey="Gervais, Patrick" sort="Gervais, Patrick" uniqKey="Gervais P" first="Patrick" last="Gervais">Patrick Gervais</name><name sortKey="Guyot, Stephane" sort="Guyot, Stephane" uniqKey="Guyot S" first="Stéphane" last="Guyot">Stéphane Guyot</name><name sortKey="Olmos, Eric" sort="Olmos, Eric" uniqKey="Olmos E" first="Eric" last="Olmos">Eric Olmos</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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